Separation of aroma compounds from industrial hemp inflorescences (Cannabis sativa L.) by supercritical CO2 extraction and on-line fractionation

The use of supercritical carbon dioxide (Sc-CO2) extraction at 10 and 14 MPa and 40 \ub0C and on-line fractionation using two separators (Sep 1: 7 MPa/25 \ub0C; Sep2: 5 MPa/15 \ub0C) to recovery volatile compounds from the inflorescences of fiber type Cannabis sativa L. was investigated by HS-SPME/GC\u2013MS and direct GC\u2013MS and compared with hydrodistillation. The best results were obtained by Sc-CO2 extraction carried out at 10 MPa and 40 \ub0C. Under these operating conditions, cuticular waxes covering the surface of flowers were collected in the first separator and volatile compounds (100%) in the second. The superior quality of this last extract was proved by the perfect overlapping of its HS-SPME/GC\u2013MS volatile profile to that of inflorescences. The recovery of fractions with different composition and biological properties, made the inflorescences of fiber type Cannabis sativa L suitable for cosmetic and/or food industry

Application of a supercritical CO2 extraction procedure to recover volatile compounds and polyphenols from Rosa damascena

A supercritical CO2 (Sc-CO2) extraction procedure to recover volatile compounds and polyphenols from Rosa damascena is investigated. It consists of two steps: the first by Sc-CO2 at 16 MPa and 313.15 K and on-line fractionation using two separators (S1: 7 MPa/ 298.15 K; S2: 5 MPa/ 288.15 K) for volatile compounds, the second by Sc-CO2 added with 10% ethanol-water mixture (57% v/v) at 8 MPa and 313.15 K for polyphenols. Sc-CO2 extract obtained in S2 resulted of high quality compared with essential oil. Polyphenol yield by SC-CO2 added with co-solvent resulted about 80 % of methanol extraction (3250 mg GAE/100 g dw)

Improved Sustainability in Wine Industry Byproducts: A Scale-up and Economical Feasibility Study for High-Value Compounds Extraction Using Modified SC-CO2

[Image: see text] The objective of the present work was to optimize the operating conditions (P, T cosolvent %) and to study the scale-up and the feasibility of the supercritical fluid extraction (SFE) process for polyphenols from grape pomace, the main solid byproduct of the wine industry. Pilot-scale equipment (1 L extraction vessel) was used to study the scale-up prediction for extraction vessels of 50, 100, 500, and 1000 L capacity. The adopted scale-up criteria consisted of maintaining and keeping constant the solvent mass-to-feed mass ratio and the bed geometry dimension. The results indicated an excellent predictive level obtained by Sovová’s model and success of the adopted scale-up criteria. At industrial scale, yields were close to 2.3 g(GAE)/100 g(DM), a value obtained using the pilot-scale equipment. High concentrations of high-added-value phenols such as cis-resveratrol glucoside, cis-coutaric acid, trans-p-coumaric acid, quercetin, and proanthocyanidins were found in the extract. An economic evaluation of the process indicated the feasibility of an industrial SFE plant with a capacity of 500 L for producing in 60 min an extract with an expected phenolics’ concentration of approximately 133 g(GAE)/kg (extract) at an estimated 67€ /kg(extract) cost of manufacturing. Notably, all values are better than those currently reported in the literature

Saúde no Brasil: a sífilis na atualidade

Monografia apresentada ao Setor de Pós-graduação da Universidade do Extremo Sul Catarinense - UNESC, para a obtenção do título de especialista em Análises Clínicas.A importância deste estudo é ressaltar a necessidade em conhecer as causas que levam o indivíduo a adquirir a Sífilis e acrescentar diferentes conhecimentos que possam servir de parâmetro para sua prevenção. Trata de uma revisão bibliográfica que teve como dados livros, sites e revistas científicas. Esta pesquisa tem como objetivo geral demonstrar as fases da doença, o diagnóstico por meio das Análises Clínicas e enfatizar a importância da prevenção como ação primordial no crescimento de casos de Sífilis, especialmente na Sífilis congênita onde infecta mais criança por transmissão vertical pelo Treponema Pallidum do que o próprio Vírus da Imunodeficiência Humana tão temida pela população. A baixa idade das primeiras relações sexuais, a variabilidade de parceiros, o não uso de preservativo e o uso de drogas ilícitas são apontados como fatores de risco às doenças sexualmente transmissíveis. Contudo, é possível notar que a Sífilis é uma patologia que deve ser prevenida, sendo extremamente curável. Dessa forma, é possível vencer o desafio e alcançar a sua erradicação

O regime diferenciado de contratações públicas: um olhar dos gestores

O presente artigo objetiva apresentar como na visão dos gestores administrativos da Universidade Federal de Santa Catarina (UFSC), se constitui o processo de desenvolvimento do Regime Diferenciado de Contratações Públicas (RDC) na Política Institucional de Licitação. A coleta de dados ocorreu por meio de entrevistas semiestruturadas e investigação bibliográfica e documental. Os resultados apontam que o RDC superou as dúvidas e incertezas inicialmente presentes na sua implantação, representou uma mudança cultural no âmbito das Licitações na UFSC, se desenvolve sob o prisma da eficácia, da celeridade processual e, ainda, propicia a devida economicidade esperada no que diz respeito à contratação de obras e serviços de engenharia

Clinical e radiographic sucess of indirect pulp capping with partial excavation of demineralization dentin in primary posterior teeth

Objective: To assess the outcome of the indirect pulp capping technique after incomplete excavation of the demineralized dentin in primary molars of patients treated in the period from 2004 to 2006.Methods: Sample consisted of 81 teeth of 36 patients aged from 1-to-8 year-old. Clinical and radiographic inclusion criteria, as well as criteria of the success of the evaluated treatments were established.Results: There were four (4.9%) failures and 77 (95.1%) successful treatments. Treatment failure occurred in three girls and in one boy, and no statistically significant difference (p=0.341) was found. Twenty three teeth were treated with resin-modified glass-ionomer cement and 58 with composite resin, of which 22 and 55, respectively, showed successful results. There was no statistically significant difference between restorative materials used (p=0.877). As regards the studied age group, patients aged from 1 to 4 years showed a significantly lower success rate when compared with 5-8 year-old children (p = 0.0428). Conclusion: The results suggest that the indirect pulp capping technique after incomplete excavation of demineralized dentin is a reliable technique to be adopted in deep lesions of primary teeth

Cuidando de mulheres com HIV/AIDS: uma análise interacionista na perspectiva de mulheres profissionais de saúde

Este estudo buscou conhecer os signifi cados atribuídos por mulheres, profissionais de saúde, ao processo de cuidar de mulheres com HIV, considerando a vulnerabilidade no contexto da feminização do HIV/AIDS. Estudo qualitativo baseado nos pressupostos da Grounded Theory e do Interacionismo Simbólico, realizado em duas maternidades públicas do Rio de Janeiro, de novembro de 2009 a abrilde 2010. Para coleta de dados utilizou-se a entrevista semiestruturada. Foram entrevistadas doze mulheres profi ssionais de saúde. Como categoria central emergiu “Falando como Profi ssional e Pensando no Cuidar”, cujo foco foi o signifi cado do cuidado, integrando duas categorias: a primeira traduz as inquietações do ser mulher/profi ssional cuidando de mulheres com HIV, e a segunda traz os significados dados por essas profi ssionais ao cuidado prestado às mulheres com o vírus. Conclui-se que as profi ssionais ainda trazem consigo a antiga visão do HIV/AIDS, contribuindo para o aumento da vulnerabilidade de gênero para o HIV, discriminação e preconceito.Descritores: Saúde da mulher. Síndrome de imunodefi ciência adquirida. Vulnerabilidade em saúde. Identidade de gênero

(R)-(-)-carvone and (1R, 4R)-trans-(+)-dihydrocarvone from poiretia latifolia vogel

The essential oils of Poiretia latifolia Vogel, native and cultivated leaves (Samples A and B, respectively) and native flowers (sample C), were obtained by hydrodistillation and analyzed by GC, GC/MS and chiral phase gas chromatography (CPGC). Twenty-four compounds were identified, representing 99.25, 99.26 and 99.23% of the oils, respectively. The major constituents of the oils were the monoterpenes (S)-(-)-limonene (16.05, 27.60, 15.60%, respectively), (1R, 4R)-trans-(+)-dihydrocarvone (18.05, 0.66 and 77.80%, respectively) and (R)-(-)-carvone (61.05, 64.20 and 4.50%, respectively). The essential oils were evaluated against some strains of Gram (+) and Gram (-) bacteria, and yeast, but displayed only modest antimicrobial activity

What determines mortality in malignant pheochromocytoma? : report of a case with eighteen-year survival and review of the literature

Pheochromocytoma (PCC) is a tumor derived from adrenomedullary chromaffin cells. Prognosis of malignant PCC is generally poor due to local recurrence or metastasis. We aim to report a case of malignant PCC with 18-year survival and discuss which factors may be related to mortality and long-term survival in malignant pheochromocytoma. The patient, a 45-year-old man, reported sustained arterial hypertension with paroxysmal episodes of tachycardia, associated with head and neck burning sensation, and hand and foot tremors. Diagnosis of PCC was established biochemically and a tumor with infiltration of renal parenchyma was resected. No genetic mutation or copy number variations were identified in SDHB, SDHD, SDHC, MAX and VHL. Over 18 years, tumor progression was managed with 131I-MIBG (iodine-metaiodobenzylguanidine) and 177Lutetium-octreotate therapy. Currently, the patient is asymptomatic and presents sustained stable disease, despite the presence of lung, para-aortic lymph nodes and femoral metastases. Adequate response to treatment with control of tumor progression, absence of significant cardiovascular events and other neoplasms, and lack of mutations in the main predisposing genes reported so far may be factors possibly associated with the prolonged survival in this case. Early diagnosis and life-long follow-up in patients with malignant pheochromocytoma are known to be crucial in improving survival